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Frontmatter -- Preface -- ACKNOWLEDGEMENTS -- PARTICIPANTS -- Contents -- The Gowland Hopkins Lecture: Transport of Folate and Pterin Compounds -- Chemical Aspects (1) -- Scottish International Education Trust Lecture: Synthetic Methods in Pteridine Chemistry: Some Applications to Pteridine Natural Products -- Synthesis, Chemical and Enzymatic Properties, and Therapeutic Potential of 6,6-Disubstituted Tetrahydroand Quinoid-Dihydro-Pterins -- The Structure of Quinonoid Dihydropterins [2-Amino-7,8(6H)-Dihydropteridin-4-ones] -- Some Simple s-Triazolopteridines -- Linear Tricyclic Analogs of Pteridines -- Reactions of Furazano [3,4-djPyrimidines and their Application to Pteridine Synthesis -- Syntheses of some Blocked 7,8-Dihydropteridines -- Synthesis of Chiral Reduced Pteridine Coenzymes -- Report on Discussions -- Chemical Aspects (2) -- Reactivity of Pteridines -- Synthesis and Antitumor Activity of 10-Deazaminopterins -- Synthesis and Biological Activity of 5-Deazafolic Acid and 5-Deazaaminopterin -- The Nor-Analogues of Folic Acid -- Report on Discussions -- Neurochemical and Clinical Aspects -- Metabolism of Pteridine Cofactors in Neurochemistry -- Pteridines in Cancer and Other Diseases -- Dihydropteridine Reductase Activity in Human Breast Cancer. Studies of 580 Cases and Correlations with Hormone Receptors and Cellular Typing -- A Critical Appraisal of Methods for the Quantitative Analysis of Tetrahydrobiopterin, Dihydrobiopterin and Biopterin in Human Urine, Serum and CSE -- Inhibition of Pterin Biosynthesis in the Adrenergic Neuroblastoma N1E115 by Tetrahydrobiopterin and Folate -- Penetration of Reduced Pterins into Rat Brain: Effect on Biogenic Amine Synthesis -- Neopterin Deficiency (GTP Cyclohydrolase I Deficiency), a New Variant of Hyperphenylalaninemia -- Folate Pathways in Cells from Fragile X Syndrome Patients and Carriers -- Report on Discussions -- Enzymology -- The Interaction of Substrates and Inhibitors with Dihydrofolate Reductase -- Catalytic Site of Dihydrofolate Reductase -- Structure, Function and Affinity Labelling Studies of Dihydrofolate Reductase -- Methylene Tetrahydrofoíate Reductase: Studies in a Human Mutant and Mammalian Liver -- Studies on Methylenetetrahydrofolate Reductase from Pig Liver: Catalytic Mechanism and Regulation by Adenosylmethionine -- Role of Methylenetetrahydrofolic Acid Reductase in Regulation of Folic Acid Metabolism and its Relation to the Methyl Trap Hypothesis -- The Mechanism of Action of the Transformylase Enzymes -- Folypolyglutamate Synthesis in Neurospora Crassa -- Report on Discussions -- Clinical Aspects: Cancer -- Folates and Cancer Chemotherapy -- Inability to Increase the Mobilisation of Liver Folates During Folate Deficiency Suggesting that this and Other Organs do not act as a Potential Folate Store for Marrow and Other Rapidly Proliferating Cells -- A High Molecular Weight Nonfunctional Immunoreactive Form of Dihydrofolate Reductase in L1210 Leukaemia Cells -- Further Characterization of the Pterdine Binding Variant of Alphas-Acid Glycoprotein, Accumulated in the Blood of Patients with Malignant Diseases -- Structure-Activity Relationships among 5-Substituted Lipophilic Diaminopyrimidine Antineoplastic Antifolates -- Attempts to Design Inhibitors of Dihydrofolate Reductase Using Interactive Computer Graphics with Real Time Energy Calculations -- Control of Methotrexate Polyglutamate Synthesis in Cultured Rat Hepatoma Cells -- Differential Effects of Methotrexate or Fluorodeoxyuridine upon Mitochondrial and Cellular Nucleotide Pools -- Report on Discussions -- Bioorganic Chemistry and Enzymology -- Chemical Mechanisms in the Action of Pteridine Coenzymes -- Irreversible Inactivation of Rat Liver Phenylalanine Hydroxylase by Reaction with (6S)-L-Erythro-Tetrahydrobiopterin -- p-Azidophenylalanine: A Potential Photoaffinity Probe for Rat-Liver Phenylalanine Hydroxylase -- Pyrimidine Models for the Cofactor of Phenylalanine Hydroxylase -- Activation of Molecular Oxygen by Tetrahydropterin Monooxygenases -- Mechanistic Studies on Phenylalanine Hydroxylase: Structural Determination of the Tetrahydropterin Intermediates -- Properties of Thymidylate Synthase from Streptococcus Faecium -- Cloning the Gene for Eukaryotic C1-THF Synthase -- Report on Discussions -- Biochemistry and Biology -- The Enzymatic Synthesis of the Drosopterins: Identification of a Pyrimidodiazepine as an Intermediate -- The Common Precursor of Sepiapterin and Drosopterin in Drosophila: Enzymatic and Chemical Synthesis -- The Common Precursor of Sepiapterin and Drosopterin in Drosophila: Enzymatic and Chemical Synthesis -- The Role of Pteridines in Regulation of Growth -- Evidence for a Folate Bound to Rat Hepatic Uroporphyrinogen III Cosynthase and its Role in the Biosynthesis of Heme -- Report on Discussions -- Presented Papers -- Aerobic Oxidation of 5,6,7,8-Tetrahydroneopterin -- The Conformations of 7,8 (6H)-Dihydropterin Substrates and the Activity of Dihydropteridine Reductase (E.C.1.6.99.10) -- Dihydrofolate Reductase: The Stereochemistry of Inhibitor Selectivity -- Synthesis of an 8-Deaza Analog of the Intermediate in the Thymidylate Synthetase Reaction (1) -- The Synthesis of Analogs of Pteroylglatamyl-y-Phosphate as Potential Inhibitors of Folylpolyglutamate Synthetase -- Synthesis and Activity of 8,10-Dideazaminopterin -- CNDO/2 Molecular Orbital Calculations on the Antifolate DAMP and Some of its Analogues: Conformational Characteristics -- Fluorescent Analogues of Methotrexate as Probes for Folate Antagonist Molecular Receptors -- Dihydrofolate Reductase Overproduction Identified by Flow Cytometry Using a Fluorescent Methotrexate Analogue -- New Syntheses and Transformations of Some Pteridines and Pteridine 3-Oxides -- Photooxidation of Mercaptopteridines -- Reactivity of 6,7-Dichloro-1, 3-Dimethyllumazine -- Synthesis, Reactivity and Properties of 8-Substituted 2-Thio- and 2,4-Dithiolumazines -- The Activity of Sulfonamide-Substituted Benzylpyrimidines Against Dihydropteroate Synthase, Dihydrofolate Reductase, and Bacterial Cell Cultures -- Structural Studies of Antifolate Drugs -- Isolation and Characterization of a Novel Folate Antagonist -- Synthesis and Biological Properties of the S-8 Analog of 7,8-Dihydropteridinealcohol -- Synthesis of Dihydrobiopterin from Dihydroneopterin Triphosphate in Rat Tissues and Human Blood Corpuscles: Diagnosis of Atypical Phenylketonuria due to Biopterin Deficiency by Assay of an Enzyme Involved in the Synthesis -- Stereospecific Enzyme Mediated Syntheses of Tetrahydrofolate Derivatives -- Immobilization of Dihydrofolate Reductase from Amethopterin Resistant Lactobacillus Casei -- X-Ray Studies of the Binding of Trimethoprim, Methotrexate, Pyrimethamine and Two Trimethoprim Analogues to Bacterial Dihydrofolate Reductase -- A New Spectrophotometry Method for the Assessment of Tight Binding Benzyldiaminopyrimidine Inhibitors of Dihydrofolate Reductase -- Two Interconverting Conformers of a Drug-Receptor Complex: The Lactobacillus Casei Dihydrofolate Reductase-NADP+ - Trimethoprim Complex -- Multiple Conformations of the Dihydrofolate Reductase- Folate-NADP+ Complex -- The Three-Dimensional Structure of Mouse L1210 Dihydrofolate Reductase -- Isolation and Amino Acid Sequence Studies of Dihydrofolate Reductase from Neisseria Gonorrhoeae -- Purification of 7,8-Dihydropteroate-Synthetase from E. Coli by Affinity- and Hydrophobic Interaction Chromatography -- Isolation and Characterization of an E. Coli Mutant Affected in Dihydrofolate- and Folylpolyglutamate- Synthetase -- Purification and Properties of 5-Formyltetrahydrofolate Cyclodehydrase from Rabbit Liver -- Structural Studies on Formyl-Methenyl-Methylene Tetrahydrofolate Synthetase from Rabbit Liver -- Utilization of Folate Polyglutamate for Growth by Lactobacillus Casei -- Folate and Folate Enzymes as Structural Components of Bacteriophage Particles -- Studies on the Substrate Specificity of Mammalian Folylpolyglutamate Synthetase -- Properties of Corynebacterium Species Dihydrofolate Synthetase-Folylpolyglutamate Synthetase -- Purification and Characterization of Folylpolyglutamate Synthetase from Lactobacillus Casei and Hog Liver -- The Molybdenum Cofactor from Xanthine Oxidase: Quantitative Assay of the Molybdenum Cofactor by Activation of Nitrate Reductase Activity in Extracts of the Nit-1 Mutant of Neurospora crassa -- Structural Studies of Calf Thymus Thymidylate Synthase -- A Comparison of the T-Even Phage Induced and Escherichia Coli Thymidylate Synthases -- Thermophilicity of Dihydrofolate Reductase and Thymidylate Synthetase from a Thermophilic Microorganism -- Chemical Characterization of Two Folate-Binding Proteins from Mitochondria: Dimethylglycine Dehydrogenase and Sarcosine Dehydrogenase -- Allosteric Mung Bean (Vigna radiata) Serine Hydroxylmethyl-Transferase -- Comparative Study of the Eye Colour Mutants of Drosophila Melanogaster: Quantification of the Eye-Pigments and Related Metabolites -- Studies on Sepialumazine, the Characteristic Larval Integument Pigment of the Bombyx Mori Kiuki Mutant -- Macromolecular Regulation of Sepiapterin and Drosopterin Synthesis in the Purple Mutant of Drosophila -- The Hydroxylation of Phenylpropanoids by Euglena gracilis : Role of Pteridines -- Studies with Old Yellow Enzyme, Reconstitution with Lumazine Analogs as Coenzyme -- Biosynthesis of Riboflavin -- Biosynthesis of Riboflavin -- Metabolism and Function of Pterin Compounds in Seed Germination -- Thymidylate Synthetase During Proliferation and Differentiation of Physarum polycephalum -- Influence of Vitamin B12 on Folate Metabolism in Lactobacillus Leichmannii -- High-Affinity Binding of Folate to a Small and Large Molecular Size Protein from Human Milk -- High-Affinity Folate Binding in Leukocytes from Normal Subjects Displays Positive Cooperativity, and the Affinity Depends on the Protein Concentration -- Characteristics of 5-Methyltetrahydrofolate Binding to the Folate-Binding Protein from Cow's Whey -- Interactions of Folate-Dependent Enzymes of DNA Precursor Metabolism in T4 Phage-Infected Bacteria -- The Regulation of Biopterin Biosynthesis in the Rat -- Biosynthesis of Tetrahydrobiopterin in Mammalian Tissues by De Novo and Salvage Pathways -- Perspectives on Tetrahydrobiopterin Biosynthesis in Mammals -- Human Dihydropteridine Reductase (DHPR) Deficiency -- Coordinate Regulation of Guanosine Triphosphate Cyclohydrolase and Tetrahydrobiopterin in Adrenal Medullary Chromaffin Cells -- Regulation of Adrenocortical Guanosine Triphosphate Cyclohydrolase and Tetrahydrobiopterin in Normal and Spontaneously Hypertensive Rats -- Inhibition of Brain Sepiapterin Reductase by a Catecholamine and an Indoleamine -- Mouse Mastocytoma Tryptophan Hydroxylase: Role of Iron and Catalase in the Expression of Enzyme Activity after Activation by Anaerobic Preincubation with Dithiothreitol -- Folic Acid Induced Kindling in Rats: Changes in Brain Amino Acids -- Impaired Pteroylpolyglutamate Synthesis in the Cobalamin-Inactivated Rat -- A Radioimmunoassay for Determination of D-Erythro- Neopterin -- Radioimmunoassay for Neopterin in Body Fluid and Tissues -- A Specific Antiserum Against Tetrahydrobiopterin: Its Preparation and Application to Immunohistochemistry -- Speculation on the Mechanism of Therapeutic Action of Tetrahydrobiopterin in Human Disease -- Correlation of Tetrahydrobiopterin Content and Guanosine Triphosphate Cyclohydrolase Activity in Cells and Tissues -- Unconjugated Pteridines in Human Physiological Fluids. Normal and Pathological Profiles -- Biopterin and Phenylalanine Metabolism During Early Liver Regeneration -- GTP-Cyclohydrolase Activities in Rat Liver -- Phenylalanine Hydroxylase Assay Using Biopterin and Synthetic Pteridine -- Dihydropteridine Reductase Levels in Human Normal and Neoplastic Tissues -- Investigations of Tissue Folates in Normal and Malignant Tissues -- Blood Cell Biopterin as an Indicator of Transplanation- Induced Hemopoiesis and Leukemic Cell Proliferation -- Assessment of Urinary Neopterin in the Early Diagnosis of Human Allograft Rejection -- Behaviour of Neopterin Levels in Patients with Haematological and Gynaecological Neoplasms -- Pteridines in Dictyostelium discoideum and Physarum polycephalum -- Biochemical and Chemotherapy Studies on BW 301U, a Novel Lipophilic 2,4-Diaminopyridopyrimidine Antifolate -- Toxicity of a Series of Antifolate Compounds in a Methotrexate-Resistant Celi Line -- Interaction of Methotrexate Poly(L-Lysine) with Transformed Hepatic Cells in Culture -- A Novel Technique for Producing Folate-Deficient Hepatica Cells -- Methotrexate Metabolism in Acute Lymphoblastic Leukemia -- Altered Methotrexate Sensitivity in Human Leukemic K562 Amino Acid Transport Mutant Cells -- Methotrexate and Methotrexate Polyglutamates in Erythrocytes After High-Dose Methotrexate -- Methotrexate Polyglutamates in Human Fibroblasts: Reversal of Antifolate Cytotoxicity -- Human Cells Resistant to Methotrexate: Cross Resistance and Collateral Sensitivity to the Nonclassical Antifolates Trimetrexate, Metoprine, Homofolic Acid and CB3717 -- Homology of Two Forms of Dihydrofolate Reductase in Human Leukaemic and L1210 Cells During Expression of Resistance to Methotrexate -- Adaptation of Folylpolyglutamates to Folate Deprivation in Mouse Hepatoma Cells -- Utilization and Loss of Reduced Folates by Human Fibroblasts in Vitro -- Factors Affecting Folate Polyglutamate Biosynthesis in Rat Liver -- Differential Binding of Folates but not Methotrexate (MTX) by Brush Border (BB) and Basal Lateral (BL) Membranes of Rat Renal Cortex -- Biosynthesis of Folate Derivatives in Ehrlich Ascites Carcinoma Cells Grown in Mice and in some Organs of the Host -- Variability of Thymidylate Synthetase in Ehrlich Carcinoma Cells and its Role in Resistance to 5-Fluorodeoxyuridine -- One-Carbon Metabolism in Human Lymphocytes -- Possible Mechanisms of Folate Catabolism -- The Intracellular Folate Pool: Studies of Kinetics and Functional Significance -- Intestinal Folate Transport. A pH-Dependent, Carrier- Mediated Process -- Effects of Folate Depletion on Growth and Survival of Human Cell Line K-562 in Culture -- Alterations in Folate Metabolism of the Rat Induced by Acute Ethanol Administration -- Participation of Pterins in the Control of Lymphocyte Transformation and Lymphoblast Proliferation -- The Pterin Component of the Molybdenum Cofactor (Molybdopterin): Relationship to Urothione -- Report on Presented Papers -- Author Index -- Subject Index -- Backmatter